Mesorhabditus monhystera
[ref. ID; 1767]
Test system
22-days food chain assay
Strain
From the Department of Nematology, Agricultural University in Wageningen, mixed culture of gutbacteria, pregnant worm.
Toxicants
CdCl2, Pb(NO3)2, and CdCl2+Pb(NO3)2
Temperature
25 degrees C
Test design
Petri dishes (wateragar + bacteria (uptaking heavy metals) + 5 worms): Bacteria suspensions were incubated for 2.5 hrs in salt solutions (0, 20, 200, and 1000 ug/g Pb(NO3)2: 2, 20, and 200 ug/g CdCl2: 20/2, 200/2, and 1000/100 ug/g in combined Pb/Cd).
Measurements/observations
Numbers of nematoda.