Cognettia sphagnetorum (Vejdovsky)
[ref. ID; 6014]
Test system
Soil microcosms with a patchy Cu-contamination
Sampling sites
- Polluted site: From a pine forest soil in Harjavalta (southwestern Finland). The area is mainly polluted by Cu from a Cu-Ni smelter. Average Cu content at the site was 2,000 mg/kg of dry soil, and bioavailable Cu content was 150 mg/kg of dry soil.
- Unpolluted site: Forest stand near the city of Lahti (southern Finland).
Toxicants
CuCl2
Measurements
Life-history characteristics, behavior, spatial distribution, population development.
[ref. ID; 6116]
Test system
Cu resistance in the asexual worms originating from two site (uncontaminated and contaminated)
Strains & soil samples
Collected from a pine forest in Harjavalta, southwest Finland, in October 1996. The area is mainly polluted by Cu from a Cu-Ni smelter. Metal-stressed worms were collected 2 km (Polluted sites) and reference worms (Unpolluted sites) 8 km from the smelter.
Test design
Resistance assay (the filter paper contact test (OECD, 1984)).
Individuals of similar size, body length 7-8.3 mm, width 0.15-0.3 mm and number of segments 39-44.
- Polluted sites: CuSO4 (0, 1, 1.5, 2.25, 2.8, 3.375 and 5.1 mg Cu m-2; n=5)
20+/-2 degrees C in darkness for 96 hr.
- Unpolluted sites: CuSO4 (0, 500, 1000, 1500, 2000, 2500 and 3000 mg kg-1 of dry soil; n=5)
20+/-2 degrees C in darkness for 2 weeks.
Measurements/observations
Body length and width (10th segment from the head) of adult individuals (>35 segments), number of segments, density of worms.
Evaluations
LC50-values by trimmed Spearman-Karber method.