Fridericia sp.
[ref. ID; 7179]
Test system
Effects of Acidification and liming
Strains
From the soil at the same locality.
Toxicants
- Acid rain (pH 4.4 and 3.1): Acidifying solutions were prepared from H2SO4 and HNO3, mixed in a 3:1 mass ratio of SO4(2-) to NO3(-). The mild acidification was equivalent to 1.05 kg S ha-1 year-1 and 0.23 kg N ha-1 year-1, while the strong acidification corresponded to 23 kg S ha-1 year-1, 5.03 kg N ha-1 year-1.
- Liming: CaCO3 powder 1500 and 4000 kg ha-1.
Test design
The soil (The gleyic cambisol used was taken from the 0-10 cm layer at Stromovka parc, Ceske Budejovice, Czechoslovakia) 40 g was placed in each of 32 plastic pots (100 ml volume). The soil depth in the pots was 2.8 cm and the surface area was 24.62 cm2. The bottoms of the pots were cut off and replaced by a piece of cotton cloth which was held firmly by a plastic ring. Twelve worms (average fresh body mass per individuals 4.152+/-0.220 mg) were put into each pots. Temperature 15+/-1 degrees C.
- Acidification: The acid solutions were applied as a simulated rainfall of 32 mm over the experimental period of 52 days.
- Liming: On the 27th day of incubation the pots were limed once. Total experimental period of 52 days.
Measurements/observations
Body weight. The activities of amylase, xylanase, trehalase, and C1-cellulase in the gut.