Euplotes
- Euplotes aediculatus
- Euplotes affinis
- Euplotes eurystomus
- Euplotes moebiusi
- Euplotes mutabilis
- Euplotes patella
- Euplotes vannus
[ref. ID; 1876]
Test system
24, 48, 72-hr acute toxicity
Strains
From activated sludge, nonaxenic.
Toxicants
CdCl2. Cd2+ concentrations (0, 1, 2.5, 5 and 10 ppm).
Test design/concentrations
100 ml Erlenmeyer flasks (original stock culture 30 ml + fresh Cerophyl-Prescott medium 50 ml + CdCl2 solution), pH 6.9-7.2, without shaking or air sparging. x 2 replicates. Temperature 25 degrees C.
Measurements/observations
Mortality.
Evaluations
LC50
[ref. ID; 6011]
Test system
Acute toxicity (24-hr LC50)
Strains
From the aeration tank of activated sludge works designated for the treatment of domestic wastes in the district of Reggio Emilia, northern Italy.
Toxicants
Hydrated cadmium chloride, hydrated copper chloride, zinc chloride, mercury chloride.
Test design
Costar tissue culture plates with 24 wells were employed. Two replicates of 12 organisms each were run of each test concentration.
Evaluations
LC50 using the probit method.
[ref. ID; 7711]
Test system
The morphologenetic dynamics of the various monster forms
Strains
E. eurystomus were cultivated with T. pyriformis, strain GL.
Toxicants/concentrations
Heat shock. A heat shock was adiminstrered by transferring a population from room temperature to 37 degrees C until cell immobilization -i.e. 10 to 15 minutes. The following day, cells showing abnormal divisions were isolated in fresh medium.
Measurements/observations
Morphology and morphogenesis.
[ref. ID; 6111]
Test system
The acute toxicity (24-hr LC50)
Strains
The organisms was obtained, initially, from Stirone Stream (northern Italy), a freshwater environment free from known toxicants and, in particular, from heavy metals. The culture medium was constituted of boiled rice and wheat grain in 10 ml of filtered natural water. 20+/-1 degrees C, photoperiod of 16:8 hr light:dark.
Toxicant/concentrations
NiCl2/6H2O (1.12, 1.25, 1.4, 1.5 ppm).
Test design
Tissue culture plates with 24 wells, 3 replicates.
Measurements
Mortality or the survivorship.
Evaluations
LC50 using the probit method.
[ref. ID; 6934]
Test system
Detoxification
Strains
Axenic culture.
Toxicants
Cd2+ (CdCl2/2H2O), Ni2+ (NiCl2) and Zn2+ (ZnSO4/7H2O).
Test design
Uptake of heavy metals:
- Run 1. The ciliates were grown by inoculating 100 mL of Bold-basal salt medium in three 250 mL conical flasks with 10 uL of original laboratory culture (40+/-2 cells) at 25 degrees C. Zinc concentration 10 ug/mL, cadmium and nickel concentration 5 ug/ml. All cultures were incubated for 6 days, at which time 5 mL samples were removed after 0, 48, 72, 96 hr.
- Run 2. 10 L of industrial effluent with 1.5 L of 72 hr grown E. mutabilis culture, and with no E. mutabilis.
Measurements/observations
Cell number.
Evaluations
Growth rate, maximum number of protozoa.
[ref. ID; 6011]
Test system
Acute toxicity (24-hr LC50)
Strains
From the aeration tank of activated sludge works designated for the treatment of domestic wastes in the district of Reggio Emilia, northern Italy.
Toxicants
Hydrated cadmium chloride, hydrated copper chloride, zinc chloride, mercury chloride.
Test design
Costar tissue culture plates with 24 wells were employed. Two replicates of 12 organisms each were run of each test concentration.
Evaluations
LC50 using the probit method.
[ref. ID; 6111]
Test system
The acute toxicity (24-hr LC50)
Strains
The organisms was obtained, initially, from Stirone Stream (northern Italy), a freshwater environment free from known toxicants and, in particular, from heavy metals. The culture medium was constituted of boiled rice and wheat grain in 10 ml of filtered natural water. 20+/-1 degrees C, photoperiod of 16:8 hr light:dark.
Toxicant/concentrations
NiCl2/6H2O (6, 7.3, 8.5, 9.9 ppm).
Test design
Tissue culture plates with 24 wells, 3 replicates.
Measurements
Mortality or the survivorship.
Evaluations
LC50 using the probit method.
[ref. ID; 7711]
Test system
The morphologenetic dynamics of the various monster forms
Strains
Euplotes vannus complex. Several clones collected from natural populations were cultivated monoxenically on dry lettuce decoction (with sea water) inoculated with Enterobacter aerogenes.
Toxicants/concentrations
CdCl2 20 ug/ml.
Measurements/observations
Morphology and morphogenesis.