Vorticella
- Vorticella microstoma
- Vorticella similis
[ref. ID; 4989]
Test system
Potential in bioremediation
Strains
From Industrial waster water released by tanning industry near the city of Lahore (Pakistan).
Toxicants
Cu2+ (CuSO4/5H2O), Cd2+ (CdCl2), Zn2+ (ZnSO4), Cr6+ (K2Cr2O7), Pb2+ (Pb(NO3)2).
Temperature
23 degrees C.
Test design/concentrations
Bold-Basal salt medium (NaNO3 0.25g/L, CaCl2/2H2O 0.025 g/L, MgSO4/7H2O 0.075 g/L, K2HPO4 0.075 g/L, KH2PO4 0.175 g/L, NaCl 0.025 g/L, EDTA 0.05 g/L, KOH 0.031 g/L, FeSO4/7H2O 0.049 g/L, H2SO4 0.001 L/L, H3BO3 (0.01142 g/L, ZnSO4/7H2O 0.00881 g/L, MnCl2/4H2O 0.00144 g/L, MoO3 0.00071 g/L, CuSO4/5H2O 0.00157 g/L, Co(NO3)2/6H2O 0.00049 g/L, pH 7.0) + glucose 1g/L.
For Cu2+, Cd2+, Zn2+, and Cr6+ the concentration in the medium on the first day was 5 ug/mL with an increase of 5 ug/mL Cu2+ every day for 44 days for Cu2+ and Cd2+, 50 days for Zn2+, and 52 days for Cr6+. For treatment with Pb2+ the concentration in the medium on the first day was 10 ug/mL with an increase of 10 ug/mL Pb2+ every day for 55 days.
Measurements/observations
Number, activity, shape and size of the protozoans.
[ref. ID; 4568]
Test system
24 hr acute toxicity
Strains
From Culture Collection of Algae and Protozoa, CEH Windermere, Ambleside, Cumbria, UK (CCAP) CCAP 1690/2.
Toxicants
Sheep dip formulations 1) OP, Ectomort (Young's Animal Health, UK) containing 8% propetamphos as the active ingredient, 2) SP, Bayticol (Bayer, Germany) containing 6% flumethrin as the active ingredient.
Test design/concentrations
96-well microtitre plates, concentrations ranging from 0.00001% to 1% (dip formulation:total volume). Temperature 20 degrees C.
Measurements/observations
Viability by phase contrast inverted microscopy.
Evaluations
Minimum inhibitory concentration (MIC).