<Copyright(C)2004 NIES-MCTS>
Since 1 December 2004

This database was generated to assemble the microarray data that have been accumulating in our laboratory and to summarize information on experiments conducted to study the effects of environmental contaminants, e.g., dioxin-related compounds. We would like to make these microarray data available to the public to help researchers in well-understanding of the biological effects of dioxins and related compounds, especially on mammalian species. The system can be used very easily to retrieve information on genes of interest from the database, to summarize the gene groups altered among selected comparison conditions, and to show graphic data that can be used to support the work of researchers.

Important note 1:
To make the database more informative we hope to receive contributions of microarray data on the effects of environmental contaminants such as dioxins, PCBs, and other related compounds. At present we can accept only microarray data assayed by Affymetrix GeneChip MOE version 430A 2.0.

Important note 2:
You may utilize any information in this database for your research. However, please contact us before publish gene alteration data retrieved from this database, or please cite this website name as DIOXIN RESPONSIVE GENE DATABASE, NIES (DRGdb-NIES).

If you have any questions or requests, please contact Seiichiroh Ohsako, PhD, DVM, ohsako@m.u-tokyo.ac.jp, at theNational Institute for Environmental Studies.
This project was financially supported in part by the Environmental Technology Development Fund of the Ministry of the Environment, Japan.
This database was developed by applying the results of research on "Developing an advanced information management environment for the modern drug discovery processes using the genetic information", using the grant-in-aid of "Next generation software development (2003)" from the Information-technology Promotion Agency (IPA) of Japan.
The research and the development were done by NS Solutions Corporation.

The "EXPERIMENT" tool be used to list experiments registered in this database according to certain criteria, such as the compounds or animals used, and then to display a brief summary of altered gene numbers by Venn diagram, Scatter plot, etc. The "CURVE" tool can be used to retrieve experiments that show dose-response or time-course change in specified genes. It can easily give graphs of response curve.
The "GENE" tool can be used to search genes of interest in all probe sets on the microarray, and then screen experiments registered in this database, in which the gene you are searching for was altered under the condition that you specify. The "DISTRIBUTION" tool can be used to show differences in levels of expression of the genes of interest among tissues or cell-lines. The data for this purpose were obtained from control samples under relatively normal conditions.
The "COMPARISON" tool is used to compare the levels of alteration of gene expression among more than two analyses. For example, it can show the numbers of genes whose expression is induced equally under two different conditions of exposure. The "ELEMENT" tool can be used to retrieve information on xenobiotic responsive-element (XRE) in the genomic sequence of each gene corresponding to probe sets on the microarray. You can also use it to compare genomic DNA properties among selected genes in terms of XRE positions and numbers.